Journal: The journal of trauma and acute care surgery
Article Title: Effects of platelet-sparing leukocyte reduction and agitation methods on in vitro measures of hemostatic function in cold-stored whole blood
doi: 10.1097/TA.0000000000001870
Figure Lengend Snippet: Viscoelastic measures in leukoreduced WB units by agitation method over time. This figure shows viscoelastic measures of LR WB units by differing agitation method over 15 days. Agitation methods included un-rocked (blue bar; none), up and down shake (green bar), continuous mechanical horizontal agitation via flat rocker (yellow bar), or continuous end-over-end rocking via a 360° rotator (red). The viscoelastic parameters measured include the MCF measured by ROTEM (A), MA measured by TEG, (B), TEG MA-ADP (C), and TEG MA-AA (D). Median measurements of MCF, TEG-MA, TEG MA-ADP, and TEG MA-AA were compared at each time point. No differences were noted. Median measurements of MCF, TEG-MA, TEG MA-ADP, and TEG MA-AA were compared within each agitation group from Day 0 to Day 15. Differences are indicated by *p < 0.05, **p < 0.01, ***p < 0.001.
Article Snippet: Hemostatic parameters were measured using viscoelastic assays (rotational thromboelastometry-Extrinsic Screening Test (ROTEM-EXTEM) and thromboelastography (TEG) platelet mapping), impedance aggregometry (agonists—adenosine phosphate, arachidonic acid, thrombin receptor activating peptide, and collagen), and a thrombin generation assay from these whole blood units before and after filtration and on 0, 5, 10, and 15 days of storage at 4°C.
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